Scientific Publications Library
The IFR along with BBSRC is fully committed to making the outputs from its research publicly available where possible.
As a means of improving access to our scientific outputs we have created this online library. Those outputs with open access are clearly marked as such and will provide the user with either a link to a downloadable PDF or to the publisher’s version of the article.
|Green open access publications are marked by the PDF icon. Click on the publication title, or the PDF icon, and read a pre-print PDF version of the publication.||Gold open access publications have the gold open padlock icon. You can read the full version of these papers on the publishing journal’s website without a subscription.|
The creation of this publications repository was funded by BBSRC.
Impact of hydrothermal and mechanical processing on dissolution kinetics and rheology of oat ß-glucan.
Carbohydrate Polymers 166 p387-397
Publisher’s version: 10.1016/j.carbpol.2017.02.077
Oat mixed-linkage ß-glucan has been shown to lower fasting blood cholesterol concentrations due notably to an increase in digesta viscosity in the proximal gut. To exert its action, the polysaccharide has to be released from the food matrix and hydrated. The dissolution kinetics of ß-glucan from three oat materials, varying in their structure, composition and degree of processing, was investigated by incubating the oats at 37°C over multiple time points (up to 72h). The samples were analysed for ß-glucan content, weight-average molecular weight and rheological behaviour. Regardless of the materials studied and the processing applied, the solubilisation of ß-glucan was not complete. Mechanical and hydrothermal processing led to differences in the viscosity flow curves of the recovered solutions, with the presence of particulates having a marked effect. This study revealed that the structure and processing methods applied to oat materials resulted in varied and complex rheological properties, especially when particulates are present.
Interaction of quercetin and its metabolites with warfarin: displacement of warfarin from serum albumin and inhibition of CYP2C9 enzyme
Biomedicine and Pharmacotherapy 88 p 574581
Publisher’s version: 10.1016/j.biopha.2017.01.092
Flavonoids are ubiquitous molecules in nature with manifold pharmacological effects. Flavonoids interact with several proteins, and thus potentially interfere with the pharmacokinetics of various drugs. Though much is known about the protein binding characteristics of flavonoid aglycones, the behaviour of their metabolites, which are extensively formed in the human body has received little attention. In this study, the interactions of the flavonoid aglycone quercetin and its main metabolites with the albumin binding of the oral anticoagulant warfarin were investigated by fluorescence spectroscopy and ultrafiltration. Furthermore, the inhibitory effects of these flavonoids on CYP2C9 enzyme were tested because the metabolic elimination of warfarin is catalysed principally by this enzyme. Herein, we demonstrate that each tested flavonoid metabolite can bind to human serum albumin (HSA) with high affinity, some with similar or even higher affinity than quercetin itself. Quercetin metabolites are able to strongly displace warfarin from HSA suggesting that high quercetin doses can strongly interfere with warfarin therapy. On the other hand, tested flavonoids showed no or weaker inhibition of CYP2C9 compared to warfarin, making it very unlikely that quercetin or its metabolites can significantly inhibit the CYP2C9-mediated inactivation of warfarin.
International Journal of Biological Macromolecules 101 p1-8
Publisher’s version: 10.1016/j.ijbiomac.2017.03.068
Pectin and cellulose nanocrystals (CNCs) isolated from banana peels were used to prepare films. The effects of a reinforcing phase (CNCs) and a crosslinker (citric acid, CA) on properties of pectin films were studied. Glycerolplasticized films were prepared by casting, with different CNC contents (0–10 wt%), with or without CA. Overall tensile properties were improved by intermediate CNC contents (around 5 wt%). The water resistance and water vapor barrier properties were also enhanced by CNC. Evidences were found from Fourier Transform Infrared (FTIR) spectra supporting the occurrence of crosslinking by CA. Additionally, the tensile strength, water resistance and barrier to water vapor were improved by the presence of CA. The 13C ssNMR spectra indicated that both CA and CNC promoted stiffening of the polymer chains.