Scientific Publications Library
The IFR along with BBSRC is fully committed to making the outputs from its research publicly available where possible.
As a means of improving access to our scientific outputs we have created this online library. Those outputs with open access are clearly marked as such and will provide the user with either a link to a downloadable PDF or to the publisher’s version of the article.
|Green open access publications are marked by the PDF icon. Click on the publication title, or the PDF icon, and read a pre-print PDF version of the publication.||Gold open access publications have the gold open padlock icon. You can read the full version of these papers on the publishing journal’s website without a subscription.|
The creation of this publications repository was funded by BBSRC.
Interaction of quercetin and its metabolites with warfarin: displacement of warfarin from serum albumin and inhibition of CYP2C9 enzyme
Biomedicine and Pharmacotherapy 88 p 574581
Publisher’s version: 10.1016/j.biopha.2017.01.092
Flavonoids are ubiquitous molecules in nature with manifold pharmacological effects. Flavonoids interact with several proteins, and thus potentially interfere with the pharmacokinetics of various drugs. Though much is known about the protein binding characteristics of flavonoid aglycones, the behaviour of their metabolites, which are extensively formed in the human body has received little attention. In this study, the interactions of the flavonoid aglycone quercetin and its main metabolites with the albumin binding of the oral anticoagulant warfarin were investigated by fluorescence spectroscopy and ultrafiltration. Furthermore, the inhibitory effects of these flavonoids on CYP2C9 enzyme were tested because the metabolic elimination of warfarin is catalysed principally by this enzyme. Herein, we demonstrate that each tested flavonoid metabolite can bind to human serum albumin (HSA) with high affinity, some with similar or even higher affinity than quercetin itself. Quercetin metabolites are able to strongly displace warfarin from HSA suggesting that high quercetin doses can strongly interfere with warfarin therapy. On the other hand, tested flavonoids showed no or weaker inhibition of CYP2C9 compared to warfarin, making it very unlikely that quercetin or its metabolites can significantly inhibit the CYP2C9-mediated inactivation of warfarin.
International Journal of Biological Macromolecules 101 p1-8
Publisher’s version: 10.1016/j.ijbiomac.2017.03.068
Pectin and cellulose nanocrystals (CNCs) isolated from banana peels were used to prepare films. The effects of a reinforcing phase (CNCs) and a crosslinker (citric acid, CA) on properties of pectin films were studied. Glycerolplasticized films were prepared by casting, with different CNC contents (0–10 wt%), with or without CA. Overall tensile properties were improved by intermediate CNC contents (around 5 wt%). The water resistance and water vapor barrier properties were also enhanced by CNC. Evidences were found from Fourier Transform Infrared (FTIR) spectra supporting the occurrence of crosslinking by CA. Additionally, the tensile strength, water resistance and barrier to water vapor were improved by the presence of CA. The 13C ssNMR spectra indicated that both CA and CNC promoted stiffening of the polymer chains.
A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile Red
Scientific Reports 7 p44501
Publisher’s version: 10.1038/srep44501
A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.