Dr Nathalie Juge
WebsiteGut Health and Food Safety Programme
Research in my group is focused on understanding the molecular mechanisms underpinning gut bacteria-mucus interaction.
There is an emerging paradigm that mucus is critical to maintain a homeostatic relationship with our gut microbiota and that any deviations from this dynamic interaction would have major implications for health, for example in colitis, colorectal cancer etc. The main structural components of mucus are heavily O-glycosylated mucins and it is believed that these endogenous glycans provide a consistent source of nutrients and binding sites to the microbiota. However, because this is an emerging field of research and because of the difficulty associated in working with mucins, the mechanisms by which gut commensal interact with mucus have not been tackled at the molecular level.
My laboratory employs a combination of genomic, molecular and biochemical techniques to investigate the mechanisms by which microbiota bacteria recognize and degrade the complex host glycans. We seek to answer questions such as ‘Does the mucus layer drive the composition of the microbial community along the GI tract? Does impairment of the mucus layer influence, or is it influenced by, bacterial colonisation and immune status? Can we restore homeostasis by targeting the mucus layer?’
This knowledge should yield new strategies to manipulate microbiota structure-function and ameliorate diseases such as inflammatory bowel diseases, which are thought to result from imbalances in microbiota community structure.
Lactobacillus reuteri Surface Mucus Adhesins Upregulate Inflammatory Responses Through Interactions With Innate C-Type Lectin Receptors
Frontiers in Microbiology, section Microbial Immunology 8 p321
Publisher’s version: 10.3389/fmicb.2017.00321
Use of Atomic Force Microscopy to Study the Multi-Modular Interaction of Bacterial Adhesins to Mucins
International Journal of Molecular Sciences 17 p1854
Publisher’s version: 10.3390/ijms17111854
The mucin-degradation strategy of Ruminococcus gnavus: The importance of intramolecular trans-sialidases.
Gut microbes 7 p302-312
Publisher’s version: 10.1080/19490976.2016.1186334
Lactobacillus reuteri Inhibition of Enteropathogenic Escherichia coli Adherence to Human Intestinal Epithelium
Frontiers in Microbiology 7 p244
Publisher’s version: 10.3389/fmicb.2016.00244
Biochemical Society Transactions 44 p166-175
Publisher’s version: 10.1042/BST20150201
BMC Genomics 16 p1023
Publisher’s version: 10.1186/s12864-015-2216-7
Unique Organization of Extracellular Amylases into Amylosomes in the Resistant Starch-Utilizing Human Colonic Firmicutes Bacterium Ruminococcus bromii.
MBio 6 p11
Publisher’s version: 10.1128/mBio.01058-15
Discovery of intramolecular trans-sialidases in human gut microbiota suggests novel mechanisms of mucosal adaptation.
Nature Communications 6 p7624
Publisher’s version: 10.1038/ncomms8624
Genomic analysis provides clues for how gut bacteria colonise specific host animalsmore +
The discovery of unusual foraging activity in bacteria species populating our gut may explain how conditions like Inflammatory Bowel Disease (IBD) link to changes in the populations of gut bacteriamore +
Dr Nathalie Juge has received just under £490,000 to work out at the molecular level how the beneficial bacteria in our guts break down insoluble dietary carbohydrate and host glycans – carbohydrates associated with proteins in the mucus layer that lines the gut.more +
Understanding the role that the gut microbiota plays in maintaining our health needs a full understanding of exactly how these bacteria bind to their hosts. In particular, there is much interest in how bacteria proteins called adhesins associate with proteins in the mucus layer.more +
New findings from the Institute of Food Research are providing insights into the interaction between bacteria and mucins, and how the specificity of these interactions affects health.more +
Honorary Senior Lecturer, Norwich Medical School, University of East Anglia, Norwich, UK
|From 2012||Research Leader in the Gastrointestinal Health and Food Safety (GHFS) Institute Strategic Programme (ISP) at IFR, Norwich, UK|
|2008 - 2012||Deputy Head of the “Integrated Biology of the Gastrointestinal Tract” Institute Strategic Programme (GIT-ISP) at IFR, Norwich, UK|
Head of the “Glycobiome and Diet” Group at IFR, Norwich, UK
Honorary Senior Lecturer, Paul Cézanne University, Marseille, France
|2005 - 2007||
Head of the IMAG Team (Engineering and Mechanism of Action of Glycosidases) at BiosCiences (CNRS), Marseille, France
Habilitation à Diriger les Recherches (HDR) at Marseille University, France
|1999 - 2004||
Head of the “Glycosidase” Team at IFR Norwich– as senior visiting scientist
|1997 - 1999||Lecturer in Biochemistry/Molecular Biology, University Paul Cézanne Marseille|
|1996 - 1997||
Post-doctoral fellow (EU Marie Curie grant) at IFR, Norwich, UK
|1995 - 1996||Post-doctoral fellow (EU contract) at Institut de Biologia Fonamental (IBF) Universitat Autonoma de Barcelona, Spain|
|1994 - 1995||Post-doctoral fellow (EMBO grant and EU contract) at Department of Chemistry, Carlsberg Laboratory, Copenhagen, Denmark|
|1989 - 1993||PhD in Biochemistry-Molecular Biology of Nutrition “Molecular and Cellular Aspects”, Aix-Marseille III University, Marseille, France|
|1987 - 1988||M.Sc in Food Sciences at University of Montpellier II, France|
|1983 - 1987||B.Sc in Biochemistry-Molecular Biology at Faculty of Sciences, Nice, France|
Donald MacKenzie (RA)
Louise Tailford (RA)
Emmanuelle Crost (post-doc on BBSRC grant BB/L008602/1)
Devon Kavanaugh (post-doc on BBSRC grant BB/K019554/1)
Elizabeth Thursby (NRP PhD student)
Dimitris Latousakis (IFR extra PhD student)
Jemma Hardy-Goddard (Year in Industry)
Scientific organisation of international conferences: Royal Society Chemistry-Carbohydrate Group: Sugars (Norwich Institute of Food Research, 2009); European Symposium on Enzymes in Grain Processing, esEGP5 (Norwich 2008) and esEGP6 (Copenhagen 2012).
Organisation Committee of NIB BBSRC Conference Roslin Institute June 18th-20th 2013.
Regularly act as external examiner of PhD and HDR Theses in Europe: UK (University of Aberdeen 2013, Imperial College London 2013; John Innes Centre, 2013, 2012, Newcastle University, 2013, 2009), Italy (Roma, 2008), Denmark (DTU, 2013, 2011, 2010, 2009, 2006), France (University Marseille, 2005,2006; University Toulouse 2013), Belgium (KUL, 2004).
Invited annual lectures (FOOD3100/5125) at Leeds University (since 2010)
Member of BBSRC pool of experts for committee B (since 2010).
Member of BBSRC as a core member of BBSRC Research Committee D (since 2013)