• DNA microarrays and global gene expression patterns
• Genomic indexing of Salmonella and E.coli
• Salmonella virulence gene expression during infectionLag Phase
• Salmonella Infection of Chicken oviduct (SafeHouse)
• Influence of Commensal flora, immune response and behaviour on Salmonella infection (VTRI104)
• Environmental regulation of the invasion and intracellular virulence gene programmes of Salmonella: a ppGpp dependent switch

• Regulation of Salmonella SPI1 & SPI2
• Bacterial nitrate metabolism in colonisation of the mammalian gastrointestinal tract
• Analysis of factors affecting Salmonella contamination of meat during the slaughter process (Biotracer)

• "Non genetic variation" gene expression within bacterial populations (Finished)

• Nucleoid associated proteins: DNA topology and gene expression.
• Acambis project on enterotoxigenic E.coli (Finished)

Use of DNA microarrays to study global patterns of gene expression

Main researcher: Arthur Thompson; Sacha Lucchini; Yvette Wormstone

For microarray details visit the IFR microarray facility web pages at http://www.ifr.ac.uk/safety/microarrays

Presented below is a general overview on microarray technology. Use the "pause", "play" and "section bullets" buttons on the top right to freeze, resume and go to a specfic section of the presentation as needed.

 

Comparing gene expression (Type 1)

 

Comparing gene expression (Type 2)

 

Example of a type 2 experiment showing a Salmonella array .

 

Expression profile of Salmonella SPI1 genes upon induction by 0.3 M NaCl.